8P0Z
AP01-S2.3 - a variant of a redesigned transferrin receptor apical domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.990, 57.370, 39.100 |
| Unit cell angles | 90.00, 113.15, 90.00 |
Refinement procedure
| Resolution | 35.950 - 1.880 |
| R-factor | 0.2143 |
| Rwork | 0.213 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.647 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18rc1_3777) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.950 | 1.947 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.192 | 1.189 |
| Rmeas | 0.219 | 1.356 |
| Rpim | 0.104 | 0.639 |
| Number of reflections | 10690 | 1053 |
| <I/σ(I)> | 5.72 | 0.75 |
| Completeness [%] | 97.1 | 93.5 |
| Redundancy | 4.2 | |
| CC(1/2) | 0.988 | 0.522 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 11 mg/mL protein in 20 mM HEPES, pH 6.8, 150 mM NaCl mixed with 100 mM MIB buffer, pH 7.5, and 24 % PEG in a ratio 1:2 |
