8OWX
Crystal Structure of METTL6 bound to SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-21 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 43 |
| Unit cell lengths | 82.856, 82.856, 130.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.430 - 2.601 |
| R-factor | 0.2195 |
| Rwork | 0.217 |
| R-free | 0.25040 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.848 |
| Data reduction software | XDS (20210205) |
| Data scaling software | XSCALE (20210205) |
| Phasing software | PHENIX (1.16_3549) |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.430 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.201 | 4.800 |
| Number of reflections | 26988 | 2653 |
| <I/σ(I)> | 9.43 | |
| Completeness [%] | 98.4 | 83.61 |
| Redundancy | 13.6 | 13.3 |
| CC(1/2) | 0.996 | 0.143 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.1 M BisTRIS propane pH 6.5, 0.2 M sodium sulfate, 20 % PEG3350 |
