8OVY
Structure of analogue of superfolded GFP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-22 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.84 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.516, 37.721, 91.795 |
Unit cell angles | 90.00, 106.38, 90.00 |
Refinement procedure
Resolution | 88.071 - 1.537 |
Rwork | 0.186 |
R-free | 0.21450 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.789 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.071 | 1.595 |
High resolution limit [Å] | 1.537 | 1.537 |
Rmeas | 0.110 | 1.350 |
Number of reflections | 59575 | 5803 |
<I/σ(I)> | 5.1 | |
Completeness [%] | 93.1 | |
Redundancy | 3.3 | |
CC(1/2) | 0.990 | 0.340 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG 3350/PEG 1K/MPD (1:1:1) 37.5%, Bicine/Trizma 0.1 M pH 8.5, Morpheus III Alkaloids 0.8% w/v, Morpheus Alcohols 0.12 M |