8OR9
Human holo aromatic L-amino acid decarboxylase (AADC) native structure at physiological pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 107.170, 107.170, 218.981 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.130 - 1.900 |
| R-factor | 0.1917 |
| Rwork | 0.191 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.527 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.15_3459: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.450 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.106 | 0.474 |
| Number of reflections | 59247 | 8482 |
| <I/σ(I)> | 10.1 | 3 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 0.1 M Hepes, 40% PEG 200 |
