8OOD
Crystal structure of human DCAF1 WD40 repeats (Q1250L) in complex with compound 15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 81.496, 81.496, 232.723 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.259 - 1.497 |
| Rwork | 0.170 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.500 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.259 | 1.520 |
| High resolution limit [Å] | 1.497 | 1.500 |
| Rmerge | 0.091 | 1.726 |
| Rmeas | 0.097 | 1.852 |
| Rpim | 0.033 | 0.652 |
| Total number of observations | 26759 | |
| Number of reflections | 74179 | 3405 |
| <I/σ(I)> | 11.1 | 1.1 |
| Completeness [%] | 99.6 | |
| Redundancy | 8.7 | 7.9 |
| CC(1/2) | 0.997 | 0.452 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2.13M LiSO4, 0.1M Tris pH 7.5 |
