8ONK

Human insulin in complex with the analytical antibody S1 Fab and the analytical antibody HUI-001 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	278.900, 52.326, 179.504
Unit cell angles	90.00, 101.14, 90.00

Refinement procedure

Resolution	45.610 - 3.403
R-factor	0.2602
Rwork	0.258
R-free	0.31090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.414
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.610	3.525
High resolution limit [Å]	3.403	3.403
Rmerge	0.202	1.041
Rmeas	0.241	1.245
Rpim	0.129	0.675
Number of reflections	35523	3330
<I/σ(I)>	5.9	1.21
Completeness [%]	99.0	93.41
Redundancy	3.3	3.2
CC(1/2)	0.992	0.594

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M TRIS pH 7.5, 15%(w/v) PEG 6000