8ONE
Crystal Structure of full-length Human Lysyl Hydroxylase LH3 - Asp190Ser mutant - Cocrystal with Fe2+, Mn2+, UDP-Glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-16 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.775 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 97.090, 100.181, 223.794 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.440 - 2.300 |
R-factor | 0.2052 |
Rwork | 0.204 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.477 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.880 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.175 | 1.827 |
Rpim | 0.107 | 1.255 |
Number of reflections | 48804 | 4443 |
<I/σ(I)> | 5.7 | 0.5 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.6 | 5.7 |
CC(1/2) | 0.995 | 0.587 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 600 mM sodium formate, 12% poly-glutamic-acid |