8OL9

Anti-FIXa Fab in complex with human des-(Gla-EGF1) FIXa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-03
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00000
Spacegroup name	I 2 2 2
Unit cell lengths	61.440, 97.270, 366.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.980 - 2.600
R-factor	0.2695
Rwork	0.267
R-free	0.32330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.552
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.980	2.695
High resolution limit [Å]	2.600	2.602
Rmerge	0.132	0.806
Rmeas	0.148	0.931
Rpim	0.065	0.452
Number of reflections	33502	2497
<I/σ(I)>	10.62	1.28
Completeness [%]	96.2	73.6
Redundancy	4.8	3.5
CC(1/2)	0.996	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	0.5 M ammonium sulphate, 0.1 M sodium citrate, pH 5.6, !.0 M lithium sulphate