8OK9

Heterodimeric complex of Archaeoglobus fulgidus Argonaute protein Af1318 (AfAgo) with DNA and AfAgo-N protein containing N-L1-L2 domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-25
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	1.01000
Spacegroup name	P 21 21 21
Unit cell lengths	81.714, 105.893, 144.347
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.690 - 2.500
R-factor	0.201
Rwork	0.198
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.876
Data reduction software	XDS (VERSION Jun 17, 2015)
Data scaling software	Aimless (version 0.7.2 : 27/05/18)
Phasing software	MOLREP (version 6.1 : 23/01/06)
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	144.350	2.550
High resolution limit [Å]	2.500	2.500
Rmerge	0.054	0.413
Rmeas	0.058	0.446
Rpim	0.022	0.167
Number of reflections	44100	4576
<I/σ(I)>	30.2	6.6
Completeness [%]	98.1	100
Redundancy	13.3	13.7
CC(1/2)	0.999	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	280	BisTris pH 5.5 0.1 M;Ammonium acetate 0.1 M; PEG10k 8%; cryo-cooling with addition of ethylene glycol to 30 %