8OIO

Crystal structure of the kelch domain of human KLHL12 in complex with PLEKHA4 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-04
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.979499
Spacegroup name	P 1 21 1
Unit cell lengths	80.469, 73.086, 103.734
Unit cell angles	90.00, 98.81, 90.00

Refinement procedure

Resolution	79.520 - 1.954
R-factor	0.2021
Rwork	0.200
R-free	0.24860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.871
Data reduction software	XDS (Feb 5, 2021 (BUILT 20210323))
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.520	1.988
High resolution limit [Å]	1.954	1.954
Number of reflections	86247	4299
<I/σ(I)>	7.3	0.8
Completeness [%]	100.0	100
Redundancy	7	6.7
CC(1/2)	0.996	0.309

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293.15	0.1 M citrate pH 5.5, 0.4 M NaCl, 27,1% PEG8K