8ODX

Interleukin 12 receptor subunit beta-1 Fn domains in complex with antagonistic FAb4 fragment and VHH.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.915
Spacegroup name	P 41 3 2
Unit cell lengths	231.402, 231.402, 231.402
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.810 - 4.400
R-factor	0.2827
Rwork	0.280
R-free	0.31150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.824
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	231.400	5.910	4.670
High resolution limit [Å]	4.400	5.390	4.400
Rmeas	0.231	1.077	3.941
Number of reflections	13975	1790	2193
<I/σ(I)>	18.54	6.4	1.36
Completeness [%]	100.0	100	100
Redundancy	70	71.21	71.36
CC(1/2)	1.000	0.959	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.34	287	11.82% Tacsimate,10% Ethylene glycol,10 % PEG smear broad