8ODP

Structure of human RTCB with GMPCPP in complex with Archease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-10
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	98.433, 122.597, 124.759
Unit cell angles	90.00, 107.88, 90.00

Refinement procedure

Resolution	51.290 - 2.300
R-factor	0.1825
Rwork	0.181
R-free	0.21580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.674
Data reduction software	XDS (20210205)
Data scaling software	Aimless (1.12.10)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	122.600	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.095	1.161
Rpim	0.039	0.465
Number of reflections	124773	6124
<I/σ(I)>	11.4	1.5
Completeness [%]	99.8
Redundancy	7
CC(1/2)	0.998	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M potassium nitrate, 20% w/v PEG 3350