8KHV
The crystal structure of glycosaminoglycan lyase GAGase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.917, 82.096, 79.885 |
| Unit cell angles | 90.00, 106.59, 90.00 |
Refinement procedure
| Resolution | 44.010 - 1.900 |
| R-factor | 0.1602 |
| Rwork | 0.159 |
| R-free | 0.19240 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.010 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.092 | 0.206 |
| Rmeas | 0.100 | 0.230 |
| Rpim | 0.038 | 0.100 |
| Number of reflections | 86421 | 4092 |
| <I/σ(I)> | 12.9 | 6.1 |
| Completeness [%] | 98.5 | 92.04 |
| Redundancy | 6.7 | 4.9 |
| CC(1/2) | 0.992 | 0.964 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES (pH 7.0), 12% (w/v) polyethylene glycol (PEG) 6000 and 0.2 M CaCl2 |
