8KE7
Crystal structure of DNA binding and cleavage core of human topoisomerase 2-beta in a DNA binding-competent conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-17 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 100.719, 113.708, 209.267 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.830 - 2.800 |
R-factor | 0.1952 |
Rwork | 0.194 |
R-free | 0.23810 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.811 |
Data reduction software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.830 | 2.940 |
High resolution limit [Å] | 2.800 | 2.840 |
Rmeas | 0.141 | |
Rpim | 0.064 | |
Number of reflections | 58573 | 5768 |
<I/σ(I)> | 11.69 | |
Completeness [%] | 99.6 | |
Redundancy | 4.8 | |
CC(1/2) | 0.756 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | ammonium acetate, calcium chloride dehydrate, sodium cacodylate trihydrate, PEG 4000 |