8KB8

Structure of the WDR91 WD40 domain complexed with Rab7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	55.452, 81.077, 116.404
Unit cell angles	90.00, 94.41, 90.00

Refinement procedure

Resolution	48.490 - 2.490
R-factor	0.2007
Rwork	0.197
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.304
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.490	2.590
High resolution limit [Å]	2.490	2.490
Rmerge	0.055	0.630
Number of reflections	35731	3982
<I/σ(I)>	13.5
Completeness [%]	98.8
Redundancy	3.5
CC(1/2)	0.998	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	5% Tacsimate pH 7.0, 0.1 M HEPES pH 7.0, 10% Polyethylene glycol monomethyl ether 5000