8JYX
Crystal structure of the gasdermin-like protein RCD-1-1 from Neurospora crassa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.144, 91.911, 105.868 |
| Unit cell angles | 90.00, 101.38, 90.00 |
Refinement procedure
| Resolution | 45.960 - 2.350 |
| R-factor | 0.2175 |
| Rwork | 0.216 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.460 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.550 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.090 | 0.820 |
| Rmeas | 0.098 | 0.886 |
| Rpim | 0.037 | 0.465 |
| Number of reflections | 63588 | 4459 |
| <I/σ(I)> | 12.3 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.998 | 0.881 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 0.1 M Bicine pH 7.4, 11% PEG 3350, 3% (w/v) D(+)-Glucose monohydrate |
