8JWU

PHD Finger Protein 7 (PHF7) fused to UBE2D2 via a (GSGG)3 linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97957
Spacegroup name	C 1 2 1
Unit cell lengths	210.540, 102.050, 78.640
Unit cell angles	90.00, 95.92, 90.00

Refinement procedure

Resolution	29.890 - 3.580
R-factor	0.2209
Rwork	0.216
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1weq 8jws 6hpr Alphafold
RMSD bond length	0.006
RMSD bond angle	0.948
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.890	3.708
High resolution limit [Å]	3.580	3.580
Number of reflections	19600	1918
<I/σ(I)>	11.16	2.02
Completeness [%]	99.3	99.69
Redundancy	6.9	7.3
CC(1/2)	0.998	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	PEG8000, DMSO, MOPS