8JWJ

PHD Finger Protein 7 (PHF7) in complex with UBE2D2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97403
Spacegroup name	P 21 21 21
Unit cell lengths	102.304, 106.825, 145.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.350 - 2.960
R-factor	0.1959
Rwork	0.194
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1weq 6hpr AlphaFold
RMSD bond length	0.007
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.350	3.067
High resolution limit [Å]	2.960	2.961
Rmerge	0.067
Rmeas	0.075
Rpim	0.034
Number of reflections	32768	3144
<I/σ(I)>	14.5
Completeness [%]	96.5	94.39
Redundancy	4.5	4.6
CC(1/2)	0.998	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	277	MPD, Tris