8JR3
Crystal structure of Hendra Virus attachment(G) glycoprotein mutant S586N in complex with neutralizing antibody 14F8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-23 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.9789 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.843, 257.014, 193.518 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.790 - 3.220 |
R-factor | 0.2167 |
Rwork | 0.215 |
R-free | 0.24130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.413 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.790 | 3.290 |
High resolution limit [Å] | 3.220 | 3.230 |
Number of reflections | 44694 | 44663 |
<I/σ(I)> | 6.57 | |
Completeness [%] | 99.0 | |
Redundancy | 5.1 | |
CC(1/2) | 0.977 | 0.662 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | PEG3350 13.5%, 100mM pH 4.7 NaAC |