8JQW

Crystal structure of V30I mutated human transthyretin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-24
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	43.170, 85.640, 64.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.550 - 1.800
R-factor	0.1786
Rwork	0.176
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.894
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.280	1.890
High resolution limit [Å]	1.800	1.800
Rmerge	0.078	0.312
Number of reflections	22870	3273
<I/σ(I)>	15.6	5.5
Completeness [%]	99.9
Redundancy	6.9
CC(1/2)	0.997	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	PEG400, MgCl2, glycerol