8JNK
Crystal structure of human ALKBH3 bound to ssDNA through active site crosslink
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9875 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 129.460, 136.151, 203.390 |
Unit cell angles | 90.00, 107.11, 90.00 |
Refinement procedure
Resolution | 46.900 - 2.690 |
R-factor | 0.2182 |
Rwork | 0.218 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.255 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.790 |
High resolution limit [Å] | 2.690 | 5.790 | 2.690 |
Rmerge | 0.077 | 0.028 | 0.688 |
Total number of observations | 348882 | ||
Number of reflections | 92893 | 9401 | 9152 |
<I/σ(I)> | 11 | ||
Completeness [%] | 99.3 | 98.7 | 98.5 |
Redundancy | 3.8 | 3.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M NaCl, 20%PEG6000 |