8JNB
CRAF ras-binding domain chimera, ligand complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-02 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 65.019, 69.775, 39.746 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.890 - 1.620 |
| R-factor | 0.2058 |
| Rwork | 0.204 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.632 |
| Data reduction software | XDS (Feb 3, 2021) |
| Data scaling software | XDS (Feb 3, 2021) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.890 | 1.678 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.049 | 0.898 |
| Rmeas | 0.053 | 0.970 |
| Rpim | 0.020 | 0.369 |
| Number of reflections | 11698 | 1097 |
| <I/σ(I)> | 22.6 | 2.02 |
| Completeness [%] | 98.7 | 93.43 |
| Redundancy | 7.1 | 6.6 |
| CC(1/2) | 1.000 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.5M ammonium sulfate, 1.0M lithium sulfate, 0.1M sodium citrate, pH 5.6 |
