8JKQ

T95R mutant IRF4 DNA-binding domain bound to an DNA containing GACA motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-13
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9752
Spacegroup name	P 21 21 21
Unit cell lengths	53.630, 68.890, 247.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.080 - 3.090
R-factor	0.2458
Rwork	0.244
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8jkl
RMSD bond length	0.003
RMSD bond angle	0.963
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.710	3.170
High resolution limit [Å]	3.090	3.090
Rmerge	0.182	1.270
Rmeas	0.190	1.321
Rpim	0.053	0.360
Total number of observations	223083	17005
Number of reflections	17407	1281
<I/σ(I)>	10.8	2.6
Completeness [%]	99.2
Redundancy	12.8	13.3
CC(1/2)	0.997	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M sodium citrate (pH 5.0) and 10% (w/v) PEG 6000