8J54

Crystal structure of RXR/DR2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-23
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	36.239, 63.646, 244.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.790 - 2.720
R-factor	0.2225
Rwork	0.217
R-free	0.27290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.496
Data reduction software	HKL-2000
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.790	2.820
High resolution limit [Å]	2.700	2.700
Number of reflections	28366	1554
<I/σ(I)>	16.5
Completeness [%]	96.8
Redundancy	12.3
CC(1/2)	0.998	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	0.1 M Bis-Tris6.5, 0.01 mM CaCl2, 18-23% PEG2000MME