8IVW
Crystal structure of NRP2 in complex with aNRP2-10 Fab fragment
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.424, 225.164, 142.996 |
| Unit cell angles | 90.00, 91.30, 90.00 |
Refinement procedure
| Resolution | 49.650 - 3.205 |
| Rwork | 0.248 |
| R-free | 0.29410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.313 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.274 | 1.247 |
| Rmeas | 0.298 | 1.352 |
| Rpim | 0.116 | 0.517 |
| Number of reflections | 66035 | 3201 |
| <I/σ(I)> | 4.33 | 1 |
| Completeness [%] | 90.9 | 89.3 |
| Redundancy | 6.3 | 6.5 |
| CC(1/2) | 0.957 | 0.434 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 21% PEG1000, 0.1M Sodium citrate tribasic dihydrate pH 5.5, 0.05M Lithium sulfate monohydrate |
