8IOI

Crystal Structure of PadR- family transcriptional regulator Rv1176c from Mycobacterium tuberculosis H37Rv.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2023-03-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	I 2 2 2
Unit cell lengths	45.446, 119.026, 139.091
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.160 - 2.940
R-factor	0.22196
Rwork	0.217
R-free	0.27023
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.251
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	3.050
High resolution limit [Å]	2.940	2.940
Number of reflections	8284	808
<I/σ(I)>	13.19
Completeness [%]	98.5
Redundancy	8.2
CC(1/2)	0.959	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	25% PEG 400, 0.1M Tris-HCl pH 8.5, 0.1M Sodium citrate tribasic dihydrate