8IDI
Crystal structure of nanobody VHH-T71 with MERS-CoV RBD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.987 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 238.549, 46.835, 95.946 |
Unit cell angles | 90.00, 112.52, 90.00 |
Refinement procedure
Resolution | 36.551 - 1.901 |
R-factor | 0.1747 |
Rwork | 0.173 |
R-free | 0.20710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.551 | 1.969 |
High resolution limit [Å] | 1.901 | 1.901 |
Number of reflections | 77670 | 7647 |
<I/σ(I)> | 21.16 | |
Completeness [%] | 99.8 | |
Redundancy | 6.4 | |
CC(1/2) | 0.995 | 0.869 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 4% v/v Tacsimate, pH 6.0, 12% w/v PEG 3350 |