8IDF

Crystal structure of human TUT1 complexed with U6 snRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.27
Spacegroup name	P 65 2 2
Unit cell lengths	78.724, 78.724, 370.272
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.990 - 3.700
R-factor	0.3063
Rwork	0.305
R-free	0.33860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wu1
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.990	19.990	3.790
High resolution limit [Å]	3.700	16.530	3.700
Rmerge	0.532	0.099	4.401
Rmeas	0.538	0.101	4.453
Total number of observations	576696
Number of reflections	13674	150	975
<I/σ(I)>	8.99	35.91	1.48
Completeness [%]	99.4	95.5	93
Redundancy	42.175	36.04	42.821
CC(1/2)	0.997	0.997	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	100mM BisTris pH 6.4, 18% PEG 3350, 2% Tacsimate pH 6.0, 8% Acetonitrile