8IA5

Small peptide enhances the binding of nutline-3a to N-terminal domain of MdmX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-01
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	47.080, 47.080, 90.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.810 - 1.930
Rwork	0.210
R-free	0.28380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.454
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MrBUMP
Refinement software	REFMAC (REFMAC5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.810	5.230
High resolution limit [Å]	1.930	1.930
Rmerge	0.068
Number of reflections	8075
<I/σ(I)>	1
Completeness [%]	98.8
Redundancy	24.1
CC(1/2)		1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M HEPES pH6.0, 1.6M Ammonium sulfate