8IA3
Crystal structure of human USF2 bHLHLZ domain in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97843 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 78.847, 56.951, 232.118 |
| Unit cell angles | 90.00, 90.92, 90.00 |
Refinement procedure
| Resolution | 37.140 - 3.500 |
| R-factor | 0.2452 |
| Rwork | 0.242 |
| R-free | 0.29740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.737 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.010 | 3.830 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.170 | 0.811 |
| Rmeas | 0.200 | 0.954 |
| Rpim | 0.103 | 0.493 |
| Total number of observations | 47261 | 10960 |
| Number of reflections | 13068 | 3062 |
| <I/σ(I)> | 5.2 | 1.6 |
| Completeness [%] | 98.4 | |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.993 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | Proplex-C5 0.1M Tris, PH 8.0, 20% w/v PEG4000 |
