8I7X
Crystal structure of human ClpP in complex with ZG36
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 113.240, 96.080, 127.000 |
| Unit cell angles | 90.00, 92.72, 90.00 |
Refinement procedure
| Resolution | 30.850 - 1.990 |
| R-factor | 0.23253 |
| Rwork | 0.231 |
| R-free | 0.25974 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.110 |
| Data reduction software | autoPROC |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.850 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.178 | 1.061 |
| Number of reflections | 184458 | 13737 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M sodium bromide, 20% (w/v) polyethylene glycol 3350 |
