8I5F

Crystal structure of the DHR-2 domain of DOCK10 in complex with Cdc42 (T17N mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-16
Detector	RAYONIX MX-225
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	94.718, 95.347, 171.678
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.070 - 2.800
R-factor	0.2329
Rwork	0.226
R-free	0.29640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wm9
RMSD bond length	0.015
RMSD bond angle	2.021
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmeas	0.104	0.614
Number of reflections	35850	3500
<I/σ(I)>	15
Completeness [%]	91.6
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG 8000, sodium chloride