8I17

Structural basis for H2A-H2B recognitions by human Spt16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97849
Spacegroup name	P 1
Unit cell lengths	46.852, 49.084, 85.195
Unit cell angles	85.95, 74.31, 61.80

Refinement procedure

Resolution	40.191 - 1.980
R-factor	0.1724
Rwork	0.170
R-free	0.21110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aoi
RMSD bond length	0.006
RMSD bond angle	0.721
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (Phenix1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.010
High resolution limit [Å]	1.980	5.370	1.980
Rmerge	0.050	0.039	0.510
Rmeas	0.060
Rpim	0.033
Number of reflections	42483	2100	1896
<I/σ(I)>	24.3
Completeness [%]	94.8	93.7	86.6
Redundancy	2.9	3.1	2.5
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4,000