8HSV
The structure of rat beta-arrestin1 in complex with a rat Mdm2 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-11 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 32 |
Unit cell lengths | 80.673, 80.673, 173.555 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.340 - 3.000 |
R-factor | 0.1786 |
Rwork | 0.176 |
R-free | 0.20850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.315 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 8.130 | 3.000 |
Rmerge | 0.107 | 0.047 | 0.844 |
Rmeas | 0.113 | 0.049 | 0.887 |
Rpim | 0.035 | 0.015 | 0.274 |
Total number of observations | 266391 | ||
Number of reflections | 25258 | 1270 | 1246 |
<I/σ(I)> | 5.3 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 10.5 | 11.4 | 10.5 |
CC(1/2) | 0.994 | 0.999 | 0.930 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 1M ammonium sulfate, 0.1M HEPES pH 9.5, 5mM n-dodecyl-b-iminodipropionic acid, monosodium salt |