8HRP
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 in complex with NAD and G3P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-10-31 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.946, 119.849, 140.632 |
| Unit cell angles | 90.00, 91.27, 90.00 |
Refinement procedure
| Resolution | 32.860 - 1.990 |
| R-factor | 0.16567 |
| Rwork | 0.164 |
| R-free | 0.20065 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.519 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 1.990 | 5.430 | 2.000 |
| Rmerge | 0.994 | 0.036 | 0.275 |
| Number of reflections | 176119 | 18825 | 17428 |
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 90.7 | 97.9 | 90.1 |
| Redundancy | 1.8 | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3350, Ammonium citrate dibasic |
