8HEO

Crystal structure of SIAH1 SBD bound to Axin2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97929
Spacegroup name	P 1 21 1
Unit cell lengths	40.818, 87.932, 59.698
Unit cell angles	90.00, 101.57, 90.00

Refinement procedure

Resolution	29.200 - 2.530
Rwork	0.207
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wzz
Data reduction software	HKL-2000
Data scaling software	HKL-3000
Phasing software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.580	2.590
High resolution limit [Å]	2.530	2.530
Rpim	0.046
Number of reflections	13846	943
<I/σ(I)>	2.22
Completeness [%]	99.9
Redundancy	6.7
CC(1/2)		0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M Bis-tris propane pH 8.0, 24% PEG 6000