8HE3

Crystal structure of importin-alpha1 bound to the HIF-1alpha nuclear localization signal (delta 724-751)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2020-02-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	78.820, 89.650, 100.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.235 - 1.900
R-factor	0.1897
Rwork	0.189
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iu7
RMSD bond length	0.004
RMSD bond angle	0.760
Data reduction software	MOSFLM
Data scaling software	SCALEIT
Phasing software	MOLREP
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.240	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	56412	3558
<I/σ(I)>	11.2	1.6
Completeness [%]	99.7	99.9
Redundancy	3.6	3.7
CC(1/2)	0.998	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	ammonium sulfate, HEPES, DTT