8HE0

Crystal structure of importin-alpha1 bound to the HIF-1alpha nuclear localization signal (wild-type)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2018-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	78.750, 90.740, 97.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.070 - 1.800
R-factor	0.1836
Rwork	0.183
R-free	0.19830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iu7
RMSD bond angle	0.677
Data reduction software	MOSFLM
Data scaling software	SCALEIT
Phasing software	MOLREP
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.070	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	65167	3807
<I/σ(I)>	13	1.8
Completeness [%]	99.5	99.7
Redundancy	6.4	6.7
CC(1/2)	0.998	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	tri-sodium citrate, HEPES, DTT