8HCU

Crystal structure of BCOR/PCGF1/KDM2B complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	67.291, 67.291, 252.035
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.010 - 2.200
R-factor	0.20214
Rwork	0.200
R-free	0.23840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jh5 4hpl
RMSD bond length	0.004
RMSD bond angle	1.169
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	126.020	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.117	1.218
Rmeas	0.122	1.262
Rpim	0.033	0.329
Total number of observations	421860	37997
Number of reflections	30637	2605
<I/σ(I)>	12	2.4
Completeness [%]	100.0
Redundancy	13.8	14.6
CC(1/2)	0.998	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.2	293	0.1M magnesium acetate, 0.1M sodium citrate pH6.2, 4% PEG5000MME