8HCJ
Structure of GH43 family enzyme, Xylan 1, 4 Beta- xylosidase from pseudopedobacter saltans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2022-07-06 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.340, 89.662, 138.437 |
| Unit cell angles | 102.73, 93.98, 98.24 |
Refinement procedure
| Resolution | 134.290 - 2.566 |
| Rwork | 0.163 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nov |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.494 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 134.290 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.020 |
| Rmeas | 0.100 | 0.280 |
| Rpim | 0.070 | 0.190 |
| Number of reflections | 103346 | 5300 |
| <I/σ(I)> | 0.04 | |
| Completeness [%] | 95.0 | 97 |
| Redundancy | 1.92 | 2.01 |
| CC(1/2) | 0.940 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 18% PEG 3350, 5% PROPANOL, 0.15M IMIDAZOLE |
