8H68
Crystal structure of Caenorhabditis elegans NMAD-1 in complex with NOG and Mg(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.026, 91.867, 133.302 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.840 - 2.200 |
| R-factor | 0.1856 |
| Rwork | 0.182 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rzl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.942 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 75.640 | 75.640 | 2.060 |
| High resolution limit [Å] | 2.010 | 8.990 | 2.010 |
| Rmerge | 0.091 | 0.031 | 1.182 |
| Rmeas | 0.108 | 0.038 | 1.411 |
| Rpim | 0.059 | 0.021 | 0.759 |
| Total number of observations | 1445 | 10112 | |
| Number of reflections | 31436 | 511 | 3017 |
| <I/σ(I)> | 8.8 | 28.9 | 0.9 |
| Completeness [%] | 98.9 | 95 | 99.8 |
| Redundancy | 3.2 | 2.8 | 3.4 |
| CC(1/2) | 0.995 | 0.996 | 0.544 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M HEPES, pH 7.5, 10% (w/v) PEG 6000, 10% (v/v) MPD |
