8H5D
Crystal structure of YojK mutant in complex with UDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER D8 QUEST |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-02 |
Detector | Bruker PHOTON II |
Wavelength(s) | 1.542 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.753, 79.979, 99.244 |
Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
Resolution | 62.350 - 2.500 |
R-factor | 0.1834 |
Rwork | 0.179 |
R-free | 0.27250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vm0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.698 |
Data reduction software | XDS |
Data scaling software | SAINT (V8.38A) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 62.350 | 5.381 | 2.587 |
High resolution limit [Å] | 2.498 | 4.271 | 2.498 |
Number of reflections | 25482 | 5175 | 25482 |
<I/σ(I)> | 12.8 | ||
Completeness [%] | 99.8 | ||
Redundancy | 9.51 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M Ammonium acetate, 0.1 M HEPES (pH 7.5) and 25% (w/v) Polyethylene glycol 3350 |