8H4M
Crystal Structure of GTP-bound Irgb6_T95D mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-08 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.122, 75.750, 80.277 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.880 - 1.680 |
R-factor | 0.2069 |
Rwork | 0.206 |
R-free | 0.23020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vex |
RMSD bond length | 0.008 |
RMSD bond angle | 0.921 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20_4459: ???) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.780 |
High resolution limit [Å] | 1.670 | 1.680 |
Number of reflections | 49012 | 14962 |
<I/σ(I)> | 11.55 | |
Completeness [%] | 99.8 | |
Redundancy | 6.9 | |
CC(1/2) | 0.998 | 0.475 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Sodium sulfate, polyethylene glycol 3350 |