8GT9
Selective targeting of the Beclin 2-Atg14L coiled coil complex by stapled peptides promotes autophagy and endolysosomal trafficking of GPCRs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 289 |
| Detector technology | CCD |
| Collection date | 2016-06-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.91836 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.230, 142.780, 140.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.000 - 2.000 |
| R-factor | 0.2147 |
| Rwork | 0.214 |
| R-free | 0.23290 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.890 |
| Data reduction software | SCALEIT |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.390 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.083 | 0.282 |
| Number of reflections | 34484 | 4709 |
| <I/σ(I)> | 19.7 | |
| Completeness [%] | 90.9 | |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.6 M ammonium sulfate, 0.1 M MES, pH6.5, and 10% Dioxane. |
