8GSV

Crystal structure of human BAK in complex with the Pxt1 BH3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-05-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.987
Spacegroup name	P 1
Unit cell lengths	69.997, 108.365, 108.416
Unit cell angles	120.03, 90.02, 89.98

Refinement procedure

Resolution	46.942 - 2.200
R-factor	0.2153
Rwork	0.211
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vwz
RMSD bond length	0.011
RMSD bond angle	1.097
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.069	0.288
Number of reflections	136032	6695
<I/σ(I)>	14.4
Completeness [%]	97.4
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.1 M Sodium citrate(pH 4.8) and 17 % PEG 3000