8GRE

F-box protein in complex with skp1(FL) and substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-26
Detector	RAYONIX MX300HE
Wavelength(s)	0.900
Spacegroup name	P 21 21 21
Unit cell lengths	77.589, 150.942, 160.286
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.710 - 2.300
R-factor	0.2071
Rwork	0.206
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.430
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.710	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	84031	8307
<I/σ(I)>	21.9	3.28
Completeness [%]	99.6
Redundancy	5.3
CC(1/2)	0.998	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	200 mM Na acetate, 100 mM Na citrate pH 5.5, 10% (w/v) PEG 4000