8GQE
Crystal structure of the W285A mutant of UVR8 in complex with RUP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | I 4 |
Unit cell lengths | 173.393, 173.393, 76.737 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.010 - 2.000 |
R-factor | 0.1747 |
Rwork | 0.173 |
R-free | 0.20030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dnv |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.166 | 0.884 |
Rpim | 0.044 | 0.241 |
Number of reflections | 76777 | 7644 |
<I/σ(I)> | 23.9 | |
Completeness [%] | 100.0 | |
Redundancy | 13.8 | |
CC(1/2) | 0.957 | 0.890 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291.15 | PEG 6000, Ammonium chloride, Hexamminecobalt (III) chloride, sucrose |