8GN6

Crystallization of Sialidase from Porphyromonas gingivalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 31 2 1
Unit cell lengths	131.160, 131.160, 181.173
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.860 - 2.100
R-factor	0.17548
Rwork	0.173
R-free	0.21597
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6myv
RMSD bond length	0.007
RMSD bond angle	1.403
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.860	2.238
High resolution limit [Å]	2.100	2.100
Number of reflections	101168	101168
<I/σ(I)>	48.4
Completeness [%]	95.8
Redundancy	15.3
CC(1/2)	0.999	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287.15	1.4M Ammonium tartrate dibasic,0.1M Tris PH 7