8GJX

Structure of the human STING receptor bound to 2'3'-cUA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41
Unit cell lengths	110.879, 110.879, 35.553
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.590 - 2.600
R-factor	0.2168
Rwork	0.212
R-free	0.25420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.507
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.590	2.720
High resolution limit [Å]	2.600	2.600
Number of reflections	13744	1664
<I/σ(I)>	7
Completeness [%]	100.0
Redundancy	6.8
CC(1/2)	0.985	0.391

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Lithium Sulfate, 0.1 M Tris pH 8.5, 16% PEG 4000