8GDV
Structure of M66I mutant of disulfide stabilized HIV-1 CA hexamer in complex with CPSF6 peptide and IP6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2022-07-01 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.0722 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 133.172, 133.172, 205.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.880 - 3.300 |
| R-factor | 0.3094 |
| Rwork | 0.307 |
| R-free | 0.34730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7snq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.544 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.880 | 3.418 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rpim | 0.131 | |
| Number of reflections | 27382 | 2733 |
| <I/σ(I)> | 6.56 | 1.41 |
| Completeness [%] | 95.4 | 97.18 |
| Redundancy | 7.4 | |
| CC(1/2) | 0.987 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 0.1 M Tris hydrochloride, 11% PEG 8000, 5% Glycerol |
